Tuesday, October 27, 2015

SACCHARIDES PROPERTIES

Introduction: 
Saccharides are organic molecules consisting of C, H and O atoms. Usually the empirical formula is Cn H2n On. Are divided into three groups: Monosaccharides, Oligosaccharides, Polysaccharides.

Objectives: 
1- Identify different sugars from its properties.
2- Differentiate mono and disaccharides.
3- Understand the relation between structure and some proporties.

Procedure:
In the first part of the experiment we are going to test some physical properties of the saccharides you have in the lab: flavour, crystal structure and colour.
-FLAVOUR: put small amount of each saccharide in your hand and taste it, Is it sweet or not sweet?
-CRYSTALS: Observe a small amount of each saccharide on a clock glass under magnification.
-COLOUR: white, transparent or creamy.


TO TEST SOLUBILITY:
- Clean and dry 5 test tubes and label them: G, M, L, SU, S
-Put 5mL of water in each test tube.
-With the aid of a spatula, put a small amount of each saccharide inside the labelled test tube and test if they are soluble or insoluble.
- Observe is each saccharide forms a mixture called dissolution or a colloidal suspension.
- Finally, add 2 drops of Lugol's iodine to each test tube and test if the reaction is positive or negative. Lugol's is a solution of elemental iodine (I) and potassium iodine (KI) in water that is ise to test a saccharide. The reaction is positive when iodine reacts by turning from yellow to a purple, dark-blue/black colour.














 
Results: We can see the different test tubes we put lugol's iodine , and one is in different colour, is the  the starch (positive).

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