MITOSIS IN AN ONION ROOT

Introduction: 
In this experiment we will see the parts of the mitosi (interfase, profase, metafase, anafase and teloface) in a onion root.

Material:
- Microscope
- Slide
- Coverslip
-Dropper
-Needles emmanegades
- Watch glass
- Beaker
- Thin tongs
- lighter
- Cellulose paper

Quemical products: distilled water, Orceine A and B and normal water.
Natural products: Onion

Procedure: 

1). Put the onion in a beaker with water one week (the roots need to touch the water). 

2). When the roots grow up 3cm, cut the final 4 mm and put in the watch glass with orceine A. 

Then, you need to put in  the heater with the flame of the lighter until you see vapor (In this process the temperature can't pass the 60 C). 

3). With the tongs take the piece of the root and put in a slide and put the orceine B with the dropper. 

4). Put the coverslip and with a piece of cellulose paper you need to push and turn in the coverslide to extend de cells. 

5). Observe with the microscope with 600 increases and you will see the parts of the mitosi. 

Observations: 

In this experiment we can't see very well the different parts of the mitosis.